Estudio de la interacción de L-cisteína, L-cisteína etil éster, L-cisteína metil éster y N- acetil cisteína con monocapas de dipalmitoilfosfatidilcolina

ARIAS, JUAN MARCELO; DUPUY, FERNANDO GABRIEL; TUTTOLOMONDO, MARIA EUGENIA; DIAZ,SONIA BEATRIZ; BEN ALTABEF, AIDA

santiago del estero

Congreso; XLIV Reunión Anual de la Sociedad Argentina de Biofísica; 2015

universidad nacional de santiago del estero

J. M. Arias2; F. G. Dupuy3, M.E. Tuttolomondo1,2, S. B. Díaz1 and A. Ben Altabef1,21Instituto de Química Física. Facultad de Bioquímica,Química y Farmacia. UNT. San Lorenzo 456. T4000CAN Tucumán, R. Argentina. 2INQUINOA-CONICET-UNT.3Instituto Superior de Investigaciones Biológicas(INSIBIO), CONICET-UNT and Instituto de Química Biológica ?Dr. Bernabé Bloj?,Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI Tucumán,R. Argentina Cysteine is the aminoacid whichhas a thiol group and that takes part in a variety of biochemical reactions. In this work we studied a number of membersof the cysteine family (L-cysteine, L-cysteine ethyl ester, L-cysteine methyl esterand N-acetyl cysteine) to understand their interaction withlipid membranes. The techniqueof Langmuir monolayers wasused in order to study the surface behaviour of the different cysteinederivatives and their interaction with films made with the phospholipid DPPC.The change in the surface tension was measured whilst keeping molecular densityand lateral packing controlled. The results indicate that, although thedifferent derivatives of cysteine presented low surface activity, they wereable to favourably interact with DPPC monolayers. Also, experiments in binarymixtures indicate that the more surface active compounds stabilized the gelphase of DPPC. References 1-     M. R. Rintoul, R.D. Morero, F.G Dupuy. Colloids andSurfaces B: Biointerfaces 129 (2015) 183?190.2-     A. Bouchet, F. Lairion, E. A. Disalvo. Biochimicaet Biophysica Acta 1798 (2010) 616?623.