INQUINOA   21218
INSTITUTO DE QUIMICA DEL NOROESTE
Unidad Ejecutora - UE
artículos
Título:
Interactions of valproic acid with lipid membranes of 1,2-dimyristoyl-sn-glycero-3-phosphocholine
Autor/es:
A. BEN ALTABEF; F. CORRALES CHAHAR; C.A. GERVASI; F. CORRALES CHAHAR; C.A. GERVASI; S.B. DÍAZ; P.E. ALVAREZ; S.B. DÍAZ; P.E. ALVAREZ; A. BEN ALTABEF
Revista:
CHEMISTRY AND PHYSICS OF LIPIDS
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2019 vol. 218 p. 125 - 135
ISSN:
0009-3084
Resumen:
Abstract Important effects resulting from the interaction between valproic acid and lipid bilayers made of the phospholipid DMPC were elucidated. The studied lipid bilayer systems were prepared in two forms, as a suspension of multilamellar spherical vesicles and as planar membranes deposited on a conducting solid support. To achieve our goal we used spectroscopic methods (FTIR and Raman) to study the former system while the latter system was characterized by using electrochemical experiments (cyclic voltammetry and impedance). For both systems, in one set of experiments valproic acid was present in the solution while in a second set of experiments valproic acid was incorporated into the lipid structure. Complex effects related to Valp incorporation into the lipid vesicles were observed. As the Valp:DMPC ratio increases the phase transition temperature decreases and the phase transition becomes less marked. Moreover, for the Valp:DMPC complex species a slight decrease in the number of gauche isomers was observed relative to the number of trans isomers what corresponds to an increase in the packing density of the acylic chains. Based on derived electrical properties of the supported membranes it can be concluded that valproic acid induces the formation of pores and other defects in the film. Particularly detrimental results the action of valproic acid when it is incorporated into the membrane.