A new colorimetric method for determination of alkylresorcinols in ground and whole-cereal grains using the diazonium salt fast blue RR

DIEGO A. SAMPIETRO, MARTA A. VATTUONE, CÉSAR A.. N. CATALÁN

FOOD CHEMISTRY

Elsevier

Año: 2009 vol. 115 p. 1170 - 1174

0308-8146

A fast and inexpensive method was developed to study the content of alkylresorcinols (ARs) in ground and whole-cereal grains. This method is based on the ability of ARs to couple with Fast blue RR salt in alkaline medium, yielding colored azo-derivatives that can be quantified colorimetrically. Good linearity was observed for olivetol in the range of 1 to 10 µg (methanol solvent, ëmax = 480 nm) and 1 to 7 µg (butanol solvent, ëmax = 530 nm). Sensitivity obtained in butanol was comparable to that obtained in the Fast Blue B based method (methanol solvent, ëmax = 520 nm). In the new colorimetric method described here, incubation time was reduced to 20 min and the stability of the reaction products was as long as 3 h. The method appears promising for the analysis of 1,3-dihydroxybenzene derivatives in biological samples, especially when screening numerous samples in plant breeding and food analyses.