Inhibition of quorum sensing in Pseudomonas aeruginosa by sesquiterpene

AMAYA SUSANA; PEREIRA JOSE; BORKOSKY SUSANA; VALDEZ JC; BARDON ALICIA; ARENA MARIO EDUARDO

PHYTOMEDICINE

ELSEVIER GMBH

Año: 2012 vol. 19 p. 1173 - 1177

0944-7113

Six sesquiterpene lactones (SLs) of the goyazensolide and isogoyazensolide-type isolated from the Argentine herb Centratherum punctatum were evaluated on their ability to inhibit virulence factors of Centratherum punctatum were evaluated on their ability to inhibit virulence factors of Pseudomonas aeruginosa ATCC 27853.  Although compounds were not able to completely inhibit bacterial growth at 200 g/ml, the SLs do altered biofilm formation, elastase activity, and production of N-acyl-homoserinelactones (AHLs) which are known quorum sensing autoinducers at lower concentration. Compounds 2, 3, and 5 displayed significant inhibitory effects on P. aeruginosa biofilm formation at 0.5 g/ml being compound 3 (1.32 M) the  most potent (42%). Compounds 2, 3, 4, 5 and 6, inhibited 39, 44, 42, 32 and 35% the production of AHLs at 100 g/ml and inhibited by more than 50% the elastase activity at 0.5 g/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents. 100 g/ml and inhibited by more than 50% the elastase activity at 0.5 g/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents. 100 g/ml and inhibited by more than 50% the elastase activity at 0.5 g/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents. Centratherum punctatum were evaluated on their ability to inhibit virulence factors of Pseudomonas aeruginosa ATCC 27853.  Although compounds were not able to completely inhibit bacterial growth at 200 g/ml, the SLs do altered biofilm formation, elastase activity, and production of N-acyl-homoserinelactones (AHLs) which are known quorum sensing autoinducers at lower concentration. Compounds 2, 3, and 5 displayed significant inhibitory effects on P. aeruginosa biofilm formation at 0.5 g/ml being compound 3 (1.32 M) the  most potent (42%). Compounds 2, 3, 4, 5 and 6, inhibited 39, 44, 42, 32 and 35% the production of AHLs at 100 g/ml and inhibited by more than 50% the elastase activity at 0.5 g/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents. 100 g/ml and inhibited by more than 50% the elastase activity at 0.5 g/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents. 100 g/ml and inhibited by more than 50% the elastase activity at 0.5 g/ml. Our results clearly indicated that sesquiterpene lactones are good candidates for the development of new antimicrobial agents acting not as bactericidal but as antipathogenic agents.