Production of tannase from wood-degrading fungus using as substrate plant residues: Purification and characterization

ORDOÑEZ R.M, COLOMBO I., ALBERTO M.R, ISLA M.I.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

SPRINGER

Año: 2011 p. 5 - 9

0959-3993

In the present study Lenzites elegans, Schizophyllum commune, Ganoderma applanatum and Pycnoporus sanguineus (wood-degrading fungi) were assayed for their tannase producing potential in culture media containing plant residues or/and tannic acid as carbon source. Aspergillus niger was used as positive control for tannase production. We also carried out the isolation, purification and characterization of the enzyme from the fungi selected as the major productor. The highest fungal growth was observed in A. niger and L. elegans in the media containing tannic acid + glucose + plant residues (Fabiana densa). A. niger and L. elegans reached the highest extracellular tannase production in a medium containing tannic acid + F. densa and in a medium supplemented with glucose + tannic acid + F. densa. The produced enzyme by L. elegans was purified by DEAE Sepharose. Km value was 5.5 mM and relative molecular mass was about 163.000. Tannase was stable at a pH range 3.0 – 6.0 and its optimum pH was 5.5. The enzyme showed an optimum temperature of 60 ºC and was stable between 40 and 60 ºC. This paper is the first communication of tannase production by wood-degrading fungi. Fermentation technology to produce tannase using plant residues and xylophagous fungi could be very important in order to take advantage of plant industrial waste.