The triazolil aminoacyl (peptidyl) penicillin TAP6 inhibits cellullar adhesión and migration in a metastatic melanoma cell line

ELIZABETH BARRIONUEVO; PATRICIA G. CORNIER; CARINA M. L. DELPICCOLO; DORA B. BOGGIÁN,; ERNESTO G. MATA; LEONOR P. ROGUIN; VIVIANA BLANK

Congreso; LXI Reunión Científica Anual Sociedad Argentina de Investigación Clínica; 2016

Melanoma is a very aggressive form of skin cancer, with increased metastatic potential and high resistance to cytotoxic agents. In a previous work, we found that a penicillin derivative named TAP6, formed by a penicillin linked to the dipeptide Phe-Leu through a triazole group, was a potent antitumor agent against B16F0 murine melanoma cells. In order to study if this agent exhibits antimetastatic properties, we initially investigated the effect of TAP6 on the proliferation of different metastatic cell lines and we found that murine melanoma B16F10 cells were the most sensitive to TAP6 (IC50 7.5±2 µM). When B16F10 cells were subjected to the wound healing assay, results showed that a 5 µM concentration of TAP6 decreased the percentage of wound closure (58±6 %, 54±10 % and 42±5 %, p