Atrial Natriuretic Factor (ANF) alleviates endoplasmic reticulum stress in acute pancreatitis.

ANA PAULA COURREGES; ANA CLARA NAJENSON; MARCELO S. VATTA; LILIANA G. BIANCIOTTI

Mar del Plata

Congreso; Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIV Reu Anual de la Sociedad Argentina de Inmunología (SAI) y LXVIII Reunión Anual de la Sociedad de Farmacología Experimental (SAFE); 2016

Sociedad Argentina de Investigación Clínica

The endoplasmic-reticulum (ER) stress response constitutesa cellular process that is triggered by a variety of conditions thatdisturb proteins folding in the E􀀴. Eu􀁍aryotic cells have developedan evolutionarily conserved adaptive mechanism, the unfoldedprotein response Which aims to clear unfolded proteinsand restore ER homeostasis. Recent studies support that the ERstress is one of the earliest events triggering acute pancreatitis.Based on previous findings from our laboratory showing thatANF significantly attenuates the severity of AP by reducing trypsinogenactivation and the inflammatory response, we sought toestablish whether ANF affected ER stress in a validated AP animalmodel. AP Was induced in Sprague Dawley strain rats (200-220g) by four repetitive cerulein injections (40ug/kg). Thirty minutesbefore the first cerulein injection animals were infused with eithersaline (control) or ANF (1ug/kg/h) for 60 min. Followng euthanasiapancreatic samples were harvested for western blot analysis andtransmission electronic microscopy (TEM) studies. ChaperoneBip, the primary controller of UPR, Was overexpressed in ceruleininduced AP (p