INVESTIGADORES
BIANCIOTTI Liliana Graciela
congresos y reuniones científicas
Título:
DIFFERENCES BETWEEN URETHANE (U) AND CHLORAL HYDRATE (CH) ON PANCREATIC AND BILE SECRETION IN RATS
Autor/es:
MARIA E. SABBATINI; MYRIAN R. RODRIGUEZ; LILIANA G. BIANCIOTTI; MARCELO S. VATTA
Lugar:
Buenos Aires
Reunión:
Congreso; ADVANCES IN THE CARE AND USE OF LABORATORY ANIMALS; 2004
Institución organizadora:
Asociacion Argentina de Ciencia y Tecnología de Animales de Laboratorio
Resumen:
The comparison of findings on digestive physiology from different research laboratories is sometimes controversial due to the fact that the digestive function is modified by anesthetics. The aim of the work was to compare the effect of two different anesthetics on spontaneous as well as evoked bile and pancreatic flows. Rats were anesthetized either with CH or U. Pancreatic flow was stimulated with CCK-8 or secretin infusion whereas bile flow was stimulated by dehidrocholate (DHC). Both U and CH diminished basal as well as evoked pancreatic and bile flow as compared with conscious rats. However, spontaneous as well as CCK or secretin evoked pancreatic flow was reduced in rats anesthetized with CH as compared with U. Protein output was also more decreased in CH anesthetized rats, supporting that the effect of CH could be related to a decrease in protein output. Nevertheless no difference was observed between the two anesthetics when basal and DHC-evoked bile secretion was studied. Present results show the importance of considering the anesthetic used in a study of pancreatic secretion when comparing results from different laboratories.