INVESTIGADORES
BELMONTE Silvia Alejandra
artículos
Título:
Purification of proteins from rat sperm membranes that interact with ligands other than phosphomannosyl residues.
Autor/es:
BELMONTE, SILVIA A; SARTOR, TIRSO; BERTINI, FRANCISCO; SOSA, MIGUEL ANGEL
Revista:
ANDROLOGIA
Referencias:
Año: 2000 vol. 32 p. 115 - 118
ISSN:
0303-4569
Resumen:
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In this
study proteins were purified from rat sperm membranes which might be the high
affinity sites for ligands of epididymal fluid other than the
mannose-6-phosphate receptors. The sperm membrane proteins were solubilized and
passed over an affinity column containing epididymal fluid proteins coupled to
a matrix. Two bands in the range of 45-55 kDa were eluted from the column with
fructose-6-phosphate but not with mannose-6-phosphate. Although the molecular
weight of these proteins are similar to those of the cation-dependent
phosphomannosyl receptors they are not related. These two proteins may
correspond either to two different receptors or to forms of the same receptor
that recognize ligands from rat epididymal fluid. Sequencing and identification
of these proteins will be the aim of future studies.
