INVESTIGADORES
BELMONTE Silvia Alejandra
artículos
Título:
Compartmentalization of lysosomal enzymes in cauda epididymis of normal and castrated rats
Autor/es:
BELMONTE, SILVIA A; ROMANO, PATRICIA; SARTOR, TIRSO; SOSA, MIGUEL ANGEL
Revista:
Archives of Andrology
Editorial:
Taylor and Francis
Referencias:
Lugar: U.K; Año: 2002 vol. 48 p. 193 - 201
Resumen:
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Compartmentalization of lysosomal enzymes in
cauda epididymis of normal and castrated rats.
Belmonte SA, Romano P, Sartor T, Sosa MA.
Instituto de Histología y Embriología, Facultad de Ciencias
Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
sbelmont@fmed2.uncu.edu.ar
The
mammalian epididymis is an organ particularly rich in acid hydrolases,
consistent with a developed lysosomal apparatus. However, some of these enzymes
could also play a role in an extracellular environment, since they are actively
secreted by the epithelium. In this study the authors measured the activity of
five acid hydrolases distributed between the epithelium, fluid, small vesicles,
and spermatozoa of the rat cauda epididymis in adult rats, and compared with
that distribution under conditions of deprivation of luminal testosterone and
testicular compounds (hemicastration). Lysosomal enzymes are differently
compartmentalized in rat cauda epididymis. Most of beta-galactosidase (beta-GAL)
and aryl sulfatase (approximately 70%) were found in soluble form within the
fluid. Some 60% of N-acetyl-beta-D-glucosaminidase (beta-NAG) and
alpha-mannosidase (alpha-MAN) become transiently bound to sperm, and
beta-glucuronidase (beta-GLU) was mostly concentrated in the epithelium. After
remotion of testis this distribution changed, as the retention of alpha-MAN,
beta-GAL, beta-GLU, and beta-NAG by the epididiymal tissue increased. The
increase of beta-GLU followed an increase of synthesis of the enzyme. The
distribution of enzymes in the epididymis from the contralateral side was
similar to that in normal rats. The different roles for each enzyme in the
epididymis are discussed.
PMID: 11964212 [PubMed - indexed for MEDLINE
Arch
Androl. 2002 May-Jun;48(3):193-201.