INVESTIGADORES
BELMONTE Silvia Alejandra
artículos
Título:
Expression and binding properties of the two mannose-6-phosphate receptors differ during perinatal development in rat liver
Autor/es:
ROMANO, PATRICIA; LÓPEZ, ANA C; MARIANI, MARIA L; SARTOR, TIRSO; BELMONTE, SILVIA A; SOSA, MIGUEL ANGEL
Revista:
Biochemical and Biophysical Research Communications
Editorial:
Academic Press (Elsevier Science)
Referencias:
Lugar: U.S.A; Año: 2002 vol. 295 p. 1000 - 1006
Resumen:
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Expression and binding properties of the two
mannose-6-phosphate receptors differ during perinatal development in rat liver.
Romano PS, López AC, Mariani ML, Sartor T, Belmonte SA, Sosa
MA.
Facultad de Ciencias Médicas, Instituto de Histología y
Embriología (IHEM), Universidad Nacional de Cuyo (Argentina), Casilla de Correo
56-(5500), Mendoza, Argentina.
Mammalian
tissues express both cation-dependent (CD-MPR) and cation-independent (CI-MPR)
mannose-6-phosphate receptors, which mediate the targeting of acid hydrolases
to lysosomes. The coexistence of the two receptors in all cell types and
tissues is still poorly understood. To determine whether these receptors might
play a role in maturation, we studied their expression and binding properties
in rat liver during perinatal development. CI-MPR expression decreases
progressively from 18-day fetuses to adults, whereas the CD-MPR showed a
transient decrease in newborn and at the 5th day after birth. Immunostaining of
the tissues showed that both receptors localize to hepatocytes at all the ages
and, additionally, the CD-MPR was reactive in megakaryocytes at early stages.
Binding assays showed differences in the B(max) and K(D) values between the
ages studied. These results demonstrate that both receptors change differentially
during perinatal development, suggesting that they play distinct roles during
organ maturation.
PMID: 12127995 [PubMed - indexed for MEDLINE
Biochem
Biophys Res Commun. 2002 Jul 26;295(4):1000-6.