IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A C subunit of the NF-Y transcription factor required for bacterial
Autor/es:
MARINA BATTAGLIA, FLAVIO A. BLANCO, LUCÍA MAZZIOTA, O. MARIO AGUILAR AND MARÍA EUGENIA
Lugar:
Ginebra
Reunión:
Congreso; 9th European Nitrogen Fixation Conference; 2010
Resumen:
Common bean (Phaseolus vulgaris) establishes a nitrogen fixing association with its
partner Rhizobium etli. In this interaction, host-dependant competitiveness has been
observed in cultivars from Mesoamerican region, which are more efficiently and
preferentially nodulated by strains from the same geographical region (1). PvNF-YC was
identified in a survey of genes that are differential expressed in the Mesoamerican cultivar
with its cognate strains and encodes C subunit of the nuclear factor Y (NF-Y)
heterotrimeric transcription factor (2). RNA interference (RNAi)-mediated gene silencing
of this gene in common bean hairy roots led to a reduced nodulation phenotype and
defects in initiation/progression of infection threads. Interestingly, expression of early
nodulin genes such as RIP, ERN and ENOD40 was not affected in these roots, indicating
that PvNF-YC might act independently or downstream of these early nodulins. Contrarily,
induction of G2/S transition cell cycle genes in response to rizobia was impaired in PvNFYCPhaseolus vulgaris) establishes a nitrogen fixing association with its
partner Rhizobium etli. In this interaction, host-dependant competitiveness has been
observed in cultivars from Mesoamerican region, which are more efficiently and
preferentially nodulated by strains from the same geographical region (1). PvNF-YC was
identified in a survey of genes that are differential expressed in the Mesoamerican cultivar
with its cognate strains and encodes C subunit of the nuclear factor Y (NF-Y)
heterotrimeric transcription factor (2). RNA interference (RNAi)-mediated gene silencing
of this gene in common bean hairy roots led to a reduced nodulation phenotype and
defects in initiation/progression of infection threads. Interestingly, expression of early
nodulin genes such as RIP, ERN and ENOD40 was not affected in these roots, indicating
that PvNF-YC might act independently or downstream of these early nodulins. Contrarily,
induction of G2/S transition cell cycle genes in response to rizobia was impaired in PvNFYCRhizobium etli. In this interaction, host-dependant competitiveness has been
observed in cultivars from Mesoamerican region, which are more efficiently and
preferentially nodulated by strains from the same geographical region (1). PvNF-YC was
identified in a survey of genes that are differential expressed in the Mesoamerican cultivar
with its cognate strains and encodes C subunit of the nuclear factor Y (NF-Y)
heterotrimeric transcription factor (2). RNA interference (RNAi)-mediated gene silencing
of this gene in common bean hairy roots led to a reduced nodulation phenotype and
defects in initiation/progression of infection threads. Interestingly, expression of early
nodulin genes such as RIP, ERN and ENOD40 was not affected in these roots, indicating
that PvNF-YC might act independently or downstream of these early nodulins. Contrarily,
induction of G2/S transition cell cycle genes in response to rizobia was impaired in PvNFYC1). PvNF-YC was
identified in a survey of genes that are differential expressed in the Mesoamerican cultivar
with its cognate strains and encodes C subunit of the nuclear factor Y (NF-Y)
heterotrimeric transcription factor (2). RNA interference (RNAi)-mediated gene silencing
of this gene in common bean hairy roots led to a reduced nodulation phenotype and
defects in initiation/progression of infection threads. Interestingly, expression of early
nodulin genes such as RIP, ERN and ENOD40 was not affected in these roots, indicating
that PvNF-YC might act independently or downstream of these early nodulins. Contrarily,
induction of G2/S transition cell cycle genes in response to rizobia was impaired in PvNFYC2). RNA interference (RNAi)-mediated gene silencing
of this gene in common bean hairy roots led to a reduced nodulation phenotype and
defects in initiation/progression of infection threads. Interestingly, expression of early
nodulin genes such as RIP, ERN and ENOD40 was not affected in these roots, indicating
that PvNF-YC might act independently or downstream of these early nodulins. Contrarily,
induction of G2/S transition cell cycle genes in response to rizobia was impaired in PvNFYCRIP, ERN and ENOD40 was not affected in these roots, indicating
that PvNF-YC might act independently or downstream of these early nodulins. Contrarily,
induction of G2/S transition cell cycle genes in response to rizobia was impaired in PvNFYCPvNFYC
RNAi roots, suggesting that this gene might be required for first cortical cell divisions
that lead to the formation of initial primordia in determined nodules. In order to identify
proteins that can physically interact with this transcription factor, a yeast two hybrid (Y2H)
screening was performed using PvNF-YC as bait and a cDNA library from root tissue
inoculated with R. etli. A total of eight clones that potentially interacts with NF-YC were
isolated and sequenced. Among them, two encode receptor-like kinases and another
clone encodes a putative transcriptional regulator. Interaction of PvNF-YC with these
gene products was verified by retransformation of yeast and is being validated by coimmunoprecipitation
assays and bimolecular fluorescent complementation inR. etli. A total of eight clones that potentially interacts with NF-YC were
isolated and sequenced. Among them, two encode receptor-like kinases and another
clone encodes a putative transcriptional regulator. Interaction of PvNF-YC with these
gene products was verified by retransformation of yeast and is being validated by coimmunoprecipitation
assays and bimolecular fluorescent complementation in
Agrobacterium-infiltrated Nicotiana benthamiana leaves. Function of these PvNF-YC
interacting proteins in nodulation efficiency and bacterial infection is being evaluated by
both RNAi and overexpression. This will help to elucidate the signal transduction pathway
specifically activated in Mesoamerican common bean in response to its cognate R. etli-infiltrated Nicotiana benthamiana leaves. Function of these PvNF-YC
interacting proteins in nodulation efficiency and bacterial infection is being evaluated by
both RNAi and overexpression. This will help to elucidate the signal transduction pathway
specifically activated in Mesoamerican common bean in response to its cognate R. etliR. etli