IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NEONATAL-MOUSE MODEL TO CHARACTERIZE VACCINES AND STRATEGIES FOR OVERCOMING PERTUSSIS IN EARLY LIFE
Autor/es:
AMBROSIS, NICOLÁS; BOTTERO, DANIELA; MARTIN AISPURO, PABLO; GAILLARD, MARÍA EMILIA; ZURITA, MARIA EUGENIA; HOZBOR, DANIELA
Reunión:
Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020 LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC) LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS); 2020
Resumen:
The recent increase in pertussis cases demands more effective approaches to overcome this health problem. In parallel with maternal immunization, neonatal immunization (NI) is a strategy needing revision. Using the intranasal challenge mouse model (Balb/C), we observed that NI may improve current pertussis epidemiology. For neonates without maternal immunity, mixed vaccination schedules with commercial pertussis acellular vaccine (BoostrixTM, GSK) and our novel outer membrane vesicle (OMV)-based vaccine candidate appear to be the most appropriate schedule to induce protection in the pups. For offspring from immune mothers, to avoid blunting effect, NI should be carried out with vaccines other than those applied during pregnancy. Here we detected that after 2-dose vaccination schedules administered subcutaneously at the age of 1 week and 3weeks, the blunting effect of maternal immunity was lost, regardless of the type of vaccine used. At 7 days after the challenge (at 14 days after the last vaccine dose), any of the 2-dose schedule tested reduced at least 4 logs the B. pertussis CFU recovered from the lungs in comparison to non-immunized mice (n=6 per group, p