INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
The lncRNA POSEIDON is required for bacterial infection and the development of nitrogen fixing nodules
TRAUBENIK, SOLEDAD; ZANETTI, MARÍA EUGENIA; FERRARI, MILAGROS; BLANCO, FLAVIO A.
Congreso; Plant Biology 2020 Worldwide Summit; 2020
American Society of Plant Biology
Legumes plants have evolved the ability to establish a nitrogen-fixing symbiosis with bacteria from the genus Rhizobium. This symbiotic interaction involves the formation of a new lateral root organ, the nodule, and the suppression of the immune response that will allow rhizobia to infect the root tissue. The activation and coordination of these two genetic programs require the reprogramming of root cells for symbiosis, which is accompanied by dramatic alterations in gene expression. The association of RNAs with the translational machinery is a regulatory mechanism that contributes to this reprogramming of roots cells for symbiosis. We have shown that long non-coding RNAs (lncRNAs) are regulated at level of association to the translational machinery during the root nodule symbiosis between Medicago truncatula and Sinhorihizobium meliloti. This high throughput analysis led us to identify POSEIDON (Polysome associated symbiotically engaged intergenic down regulated long non-coding RNA) as a lncRNA that decreases its association to the translational machinery in response to rhizobia. We identified two isoforms generated from POSEIDON locus, a long isoform of and a short isoform of 450 nucleotides. Knockdown of each these isoforms affected the frequency and progression of infection events and caused significant changes in the number, development, and distribution of root nodules. Our results indicate that POSEIDON mediates the infection process as well as the development of functional nodules. This lncRNA, which is associated with the translational machinery, might act either repressing or activating the translation of its transcript targets. Alternatively, POSEIDON might encode functional small peptides that contribute to the reprogramming of root cells for the symbiotic interaction. Elucidating the mechanisms by which POSEIDON exert its activity will allow a better understanding of the biological phenomenon that leads to the biological nitrogen-fixation and transfer this information to programs for the improvement of relevant agronomic traits.