INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Unrevealing the translational reprogramming of root cells during nitrogen fixing symbiosis
TRAUBENIK, SOLEDAD; BAILEY-SERRES, JULIA; ZANETTI, MARIA EUGENIA; REYNOSO, MAURICIO ALBERTO; BLANCO, FLAVIO A.; HUMMEL, MAUREEN; HOBECKER, KAREN VANESA
Congreso; XVIII International Congress on Molecular Plant-Microbe Interactions; 2019
Legumes plants have evolved the ability to establish a nitrogen fixing symbiosis with rhizobial bacteria. The establishment of this symbiotic interaction requires the formation of a new lateral root organ, the nodule, and the suppression of the immune response that will allow the rhizobia to infect the root tissue. The activation and coordination of these two genetic programs requires the reprogramming of root cells for symbiosis, which is accompanied by dramatic alterations in gene expression. Regulation of gene expression occurs at different tiers, including transcriptional, co-transcriptional and post-transcriptional events. Our studies has been focused on the co- and post-transcriptional events including alternative splicing, translation and non-coding RNAs-mediated repression. By combining TRAP (Translating Ribosome Affinity Purification) with Ribosome footprinting we have profiled the root translatome at early stages of the legume-rhizobia symbiosis. We have found that translation of hundreds of transcripts is finely regulated and not necessarily correlated with transcription. Transcripts with altered translational status include protein-coding and non-coding RNAs, some of which exhibited cell-specific regulation. Transcript variants produced by either alternative splicing or alternative polyadenylation are also tightly regulated at translational level. Using molecular and genetic approaches we have revealed that these translational regulated transcripts play crucial role in the establishment of a successful nitrogen fixing symbiosis.