Bordetella bronchiseptica diguanilate cyclase BdcA regulates motility and is involved in transmission between hosts

MARIA DE LA PAZ GUTIERREZ; M. CARTELL; G. O'TOOLE; KEILA BELHART; NICOLÁS AMBROSIS; E. HARVILL; JULIETA FERNANDEZ; FEDERICO ZACCA; D. TAYLOR; FEDERICO SISTI

Ventura

Conferencia; Gordon Research Conferences: Signal transduction in Microbiology; 2018

Bordetella bronchiseptica causes respiratory tract infections in mammals and forms biofilm structures over abiotic surfaces or in nasal epithelium of infected mice. We previously described a diguanylate cyclase BdcA (Bordetelladiguanylate cyclase A), involved in c-di-GMP synthesis and motility regulation. In the present work we further described mechanism through this diguanylate cyclase is able to regulate motility andbiofilm.Aminoacid replacement fromBdcA active site confirmed diguanylate cyclase activity was necessary for biofilm and motility regulation. Particulalry, high c-di-GMP levels achieved by over expressing BdcA reduced significantly FlaAprotein presence in a two component system BvgAS dependent mechanism.However, motility in absence of BvgA or BvgS was inhibited by BdcA,suggesting that c-di-GMP regulates motility through more than one mechanism. In the absence of bdcA we observed enhanced motility strengthening the hypothesis that BdcAregulates motility in B. bronchiseptica. We also identified a dual GGDEF-EAL protein, BB2109, necessary for BdcA activity. This dual protein resulted important for motility and biofilm regulation by BdcA. Importantly, absence of BdcA impaired bacteria transmission between hosts. Here, we present transmission experiments that demonstrated that c-di-GMP network and particullarly BdcA is important for B. bronchiseptica pathogenesis,particularly in transmission between hosts