INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Translational reprogramming of root cells during nodule symbiosis
MARIA EUGENIA ZANETTI, SOLEDAD TRAUBENIK, KAREN HOBECKER, MAURICIO REYNOSO, MARCOS LANCIA, FLAVIO BLANCO
Congreso; International Plant Molecular Biology 2018; 2018
International Plant Molecular Biology
mRNA translation mediates developmental and adaptive responses in plants, but the impact of this level of regulation in the reprogramming of root cells during symbiosis have remained unexplored. We have found that in Medicago trauncatula roots adjustments in the translatome are poorly correlated with changes in the transcriptome. Some of the transcripts with altered translational status exhibited cell-specific regulation during symbiosis. Alternative transcript variants regulated at the translational level are enriched in chromatin remodeling, transcriptional regulation and mRNA stability. Silencing of SUPERKILLER 3, a component of the complex involved in 3´ to 5´ mRNA degradation highlights how the translatomic approach contributes to identify genes with key functions in the development of symbiotic nodules. Association of long non-coding RNAs with the translational machinery is also modulated during symbiosis, such as an alternative polyadenilated variant of the trans-acting RNA 3 (TAS3) that modulates tasiRNA biogenesis upon infection. Our results provide new insights into the translational reprogramming of gene expression during symbiosis.