INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
PROTECTIVE CAPACITY OF THE HUMORAL RESPONSE INDUCED BY A NOVEL ACELLULAR PERTUSSIS VACCINE CANDIDATE
GAILLARD ME; CARRIQUIRIBORDE F; CASTUMA C; BOTTERO D; SABATER D; RUMBO M; ZURITA E; MORENO G2; ERREA A; HOZBOR D
Simposio; 11 th Internation Bordetella Symposium; 2016
PROTECTIVE CAPACITY OF THE HUMORAL RESPONSE INDUCED BY A NOVEL ACELLULARPERTUSSIS VACCINE CANDIDATEZURITA M1; GAILLARD M1; BOTTERO D1; MORENO G2; CARRIQUIRIBORDE F1; SABATER D1; ERREA A2; CASTUMAC1; RUMBO M2; HOZBOR D11Lab. VacSal- Instituto de Biotecnología y Biología Molecular - Fac. Cs. Exactas - UNLP, 2Instituto de EstudiosInmunológicos y Fisiopatológicos - Fac. Cs. Exactas - UNLPBackgroundBased on the current epidemiologic pertussis situation, new generation of vaccines to improve the control of the disease has been proposed. In this context we have developed a vaccine based on Outer Membrane Vesicles(OMVs), derived from Bordetella pertussis. This vaccine candidate elicits protective capacity in mice model conferringlong-lasting immunity.ObjectiveCharacterize the humoral immune response induced by OMVs-based vaccine (TdapOMVBp) in comparison withcurrent commercial vaccines.Materials and MethodsTo obtain immune sera, groups of 5 mice were i.p. immunized with TdapOMVBp (3ug of protein/dose) or withcommercial wP and aP vaccines (1/10 human dose). The levels of specific total IgG and IgG1 and IgG2a isotypeswere determined by ELISA. The contribution of humoral immune response was assessed by in vivo passive transferexperiments and by in vitro opsonophagocytosis assays using RAW murine macrophages.ResultsSignificant amounts of total IgG antibodies were detected in sera from TdapOMVBp immunized mice with a highIgG2a/IgG1 ratio (1.25±0.43); suggesting a mixed Th1/Th2 profile. Interestingly, the transfer of TdapOMVBp-immunesera led to protection against B. pertussis, reducing in approximately 2.5 logs the UFC recovered from lungsof challenged mice in comparison with those recovered from non-immunized mice. In agreement with theseresults, we observed that bacteria were efficiently opsonized by TdapOMVBp-immune sera.ConclusionThe results presented here show that TdapOMVsBp elicits a humoral immune response which is involved in protectionas it is observed for current commercial vaccines.