IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
REGULATORY miRNAs INVOLVED IN THE PHASEOLUS VULGARIS - RHIZOBIUM ETLI SYMBIOSIS
Autor/es:
CASTAINGTS M, BUZZATO M, BLANCO F, ZANETTI ME.
Lugar:
Buenos Aires
Reunión:
Jornada; Codificar o no codificar: rol de ARN no codificantes en la fisiologĂ­a y la patologĂ­a; 2016
Institución organizadora:
Sociedad Argentina de Biologia
Resumen:
Phaseolus vulgaris establishes a symbiotic interaction with Rhizobium etli, resulting in the formation of root nitrogen-fixing nodules. Interestingly, plants from the Mesoamerican center of genetic diversification are more efficiently and preferentially nodulated by strains that are predominant in soils from the same geographical region. Our group has identified several genes that are involved in this preferential interaction. More recently, we have focused on the study of small non-coding RNAs (sRNAs), which are keys regulators of post-transcriptional gene expression, particularly in the establishment and regulation of nodulation. To get insight into sRNAs whose levels are modified during this preferential symbiosis, we have constructed and sequenced Illumina libraries of sRNAs from roots inoculated with either a more or a less efficient strain. More than 28 million of reads per library were obtained, filtered and mapped to the P. vulgaris genome and analyzed using the Workbench tools. We focused on the identification of microRNAs (miRNAs) that change their abundance in the more efficient interaction as compared to the less efficient one. One of them, miR390b, is a miRNA evolutionary conserved in the plant kingdom with important roles in leaf development and root architecture. Overexpression of the miR390b precursor in P. vulgaris hairy roots negatively affected nodule formation in the more efficient, but not in the less efficient symbiotic interaction. Interestingly, we have also identified two new miRNAs that are processed from the untranslated region of protein coding genes and are accumulated at higher levels in the more efficient interaction. Further characterization of these new miRNAs by functional genetic will reveal whether they play an important role in the establishment of the efficient symbiosis.