INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
IDENTIFICATION OF lncRNAs INVOLVED IN THE RESPONSE TO RHIZOBIAL SIGNALING MOLECULES
CASTAINGTS MÉLISSE; ZANETTI MARIA EUGENIA; CLAUDIO RIVERO; BLANCO FLAVIO
Jornada; XVIII Jornadas Anuales Multidisciplinarias; 2016
Common bean (Phaseolus vulgaris) establishes a symbiotic relationship with soil bacteria, resulting in the formation of root nodules in which bacteria are allocated and atmospheric nitrogen reduction takes place. In an effort to dissect the molecular responses mediated by rhizobial signaling molecules, we sought for long non-coding RNAs (lncRNAs) differentially expressed in response to rhizobial strains with different symbiotic efficiency, as well as with mutants defective in the synthesis of signaling molecules involved in the interaction. Here, we show that the percentage of lncRNAs is significantly higher in the population of transcripts differentially expressed than in the whole transcriptome of common bean. Notably, the majority of the differentially expressed lncRNAs are down-regulated in response to rhizobia. This down-regulation is mainly dependant on the Nod Factor, but also influenced by the presence of exo- and lipo-polysaccharides. In addition, we identified a group of lncRNAs differentially expressed in roots inoculated with an efficient strain of Rhizobium etli (SC15) as compared with a less efficient one (55N1). When we integrated the lncRNA data with small RNA libraries obtained from the same samples, we identified 24-mer siRNAs associated to the loci that produce these differentially expressed lncRNAs, suggesting that they might function as cis-acting modulators of transcription via chromatin remodeling. These lncRNAs will be further characterized by reverse genetics to elucidate their role in the nodulation process, and particularly, in the efficiency of the interaction in the common bean model.