INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Pangenomic relationships among Rhizobium species that nodulate and fix nitrogen in common bean Phaseolus vulgaris
PATRICIA BUSTOS; ROSA SANTAMARÍA; FABIOLA MIRANDA; DIANA GALLEGO; GONZALO A. TORRES TEJERIZO; LUIS LOZANO; OLGA PEREZ-CARRASCAL; SOLEDAD JUAREZ; MARCO A. ROGEL-HERNÁNDEZ; JOSE LUIS ACOSTA; ERNESTO ORMEÑO-ORRILLO; IRMA MARTINEZ; ESPERANZA MARTINEZ-ROMERO; MIGUEL ANGEL CEVALLOS; DAVID ROMERO; PABLO VINUESA; SUSANA BROM; VICTOR GONZALEZ
Congreso; IV Congreso de Bioquímica y Biología Molecular de Bacterias; 2015
Symbiotic nitrogenfixation by rhizobia with species of leguminous plants is widespread in nature.The promiscuous nature of common bean (Phaseolus vulgaris) hostingdifferent rhizobial species is known. R. etli, R. phaseoli, R.tropici, R. gallicum, R. giardinii, and several other species(1) have been reported as P. vulgaris nodule bacteria. Complete genomesfrom some of them are available (2,4,5), and genome comparisons have led thereevaluation of the taxonomic status of the Rhizobium species thatnodulate bean (3). Searching the genomicvariants to perform symbiosis with common bean, we determined the genomesequences of rhizobial species and their strains, which have the commonproperty of forming nodules in bean. We have sequenced the complete genome of R.gallicum R602 and IE4872, Sinorhizobium fredii GR64, R. leucaenaeNXC24, Sinorhizobium americanum NXT3, and the R. etli bvphaseoli strains IE4771, IE4803, GR56, Kim5, TAL182, CIAT894, Bra5,NXC12 and NXC14. These genomic sequences, with others previously reported (2,4, 5), allowed us to make an extended comparison of the core and accessory components;as well as, to infer the common content among them that are supposedly neededto interact with bean. The pSym plasmids display different degrees ofconservation, except for the highly conserved pSym found in R. etli and R.phaseoli. This plasmid might be of recent origin, in contrast to the pSymsof other symbiovarieties and species; as it is revealed by the variations insynonymous codons in most of their genes. Our analyses have also revealed boththe common set of symbiotic genes, as well as, a number of species-specificsymbiotic genes. Most of these later genes encode for proteins and enzymesneeded for the biosynthesis of the extracellular envelope. Specific symbioticgenes might be the basis to determine the host-range shown by the Rhizobiumspecies, analyzed here.1) C. Silva, et al. 2003. Appl EnvironMicrobiol. 69(2):884-93.2) E. Ormeño-Orrillo, et al. 2012. BMCGenomics 13: 735.3) M.G. López-Guerrero et al. 2012.Syst Appl Microbiol. 35(6):353-8.4) V. González et al. 2006. PNAS. 103(10):3834-39.5) V. González, et al. 2010. Appl. Environ. Microbiol.,76:1604?14.