A diguanilate cyclase protein from Bordetella bronchiseptica has a role in biofilm formation on lung epithelial cells.

FERNANDEZ, JULIETA; SISTI, FEDERICO

Rosario

Congreso; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014

SAIB

Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in a wide variety of host. In a previous work we described c-di-GMP second messenger role in motility and biofilm formation through overexpression of a diguanilate cyclase (BB3576).In this work, we are reporting phenotypic analysis of a BB3576 deletion mutant. C-di-GMP quantification confirmed DGC functionality (0,1977± 0,09 nM/mg in BbBB3576- vs 1,1706 ± 0,16  nM/mg in parental strain). As found in other organisms that contain low c-di-GMP levels, we observed an enhanced motility in BbBB3576-, that was restored to normal levels in the complemented mutant strain (BbBB3576-::pBBRMCS5BB3576). Surprisingly, biofilm formation on abiotic surfaces was indistinguishable from parental strain in the tested conditions. However biofilm formation on Cystic Fibrosis Bronchial Epithelial Cell Line (CFBE) showed a diminished biofilm formation by BbBB3576-, but show no differences in cell invasion. Further research will be focus to understand how motility affects bacterial ability to adhere to the epithelium, as described in other pathogens. Strategies are already designed to analyze protein interaction between BB3576 and the flagellar system.