INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles
MAXIMILIANO ORMAZABAL; BARTEL ERIKA; MARIA EMILA GAILLARD; DANIELA BOTTERO; AGUSTINA ERREA; EUGENIA ZURITA; GRISELDA MORENO; MARTIN RUMBO; CELINA CASTUMA; DARIO FLORES; DANIELA HOZBOR
Congreso; 8th Vaccine & ISV congress; 2014
Pertussis has resurged during the last two decades in different countries. In particular in 2010-2013 period large outbreaks were detected in US, Australia, UK and The Netherlands with significant mortality in infants. The epidemiological situation of pertussis point out the need to develop new vaccines and in this sense we previously developed a new vaccine based on outer membrane vesicle (OMV) which have been shown to be safe and to induce protection in mice. We have here further investigated the properties of OMV vaccines; in particular we investigated the contribution of pertussis toxin (PTx) and pertactin (Prn) in OMVs-mediated protection against pertussis. A PTx-deficient OMVs and Prn-deficient OMVs were obtained from defective B. pertussis mutants. The absence of PTx or Prn did compromise the protective capacity of the OMVs formulated as Tdap vaccine. In vivo, whereas the protective efficacy of the PTx-deficient OMVs in mice was comparable to Prn-deficient OMVs, the protective capacity of both of them was significantly impaired when it was compared with the wild type OMVs. Interestingly, using OMVs obtained from B. pertussis strain which does not express any of the virulence factors but express the avirulent phenotype; we observed that the protective ability of such OMVs was lower than that of OMVs obtained from virulent B. pertussis phase. However, it was surprising that although the protective capacity of avirulent OMVs was lower, they were still protective in the used mice model. These results allow us to hypothesize that OMVs from avirulent phase shares protective components with all OMVs assayed. Using immune proteomic strategy we identified some common components could play an important role in protection against pertussis.