Dynamics of cytoplasmic mRNAs at early stages of the nitrogen fixing symbiosis between the model legume Medicago truncatula and Sinorhizobium meliloti

MARCOS LANCIA, MAURICIO ALBERTO REYNOSO, FLAVIO BLANCO, MARÍA EUGENIA ZANETTI

Mar del Plata

Congreso; XV Congreso Latinoamericano de Fisiología Vegetal y XXX Reunión Argentina de Fisiología Vegetal; 2015

Sociedad Argentina de Fisiología Vegetal

Eukaryotic mRNAs associate to RNA-binding proteins that influence their stability, transport, cellular localization and translatability. In the cytoplasm, mRNAs can be translated into polysomes, targeted for degradation into P-bodies or stored in cytoplasmic granules. We have genome-wide characterized changes in the association of mRNAs to polysomes in roots of the model legume Medicago truncatula at early stages of the symbiotic interaction with Sinorhizobium meliloti using Translating Ribosomes Affinity Purification (TRAP) combined with RNA -Seq technology. This analysis identified a number of mRNAs that do not change in total abundance, but are significantly up- or down- regulated at the level of association with polysomes.