IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lap proteins are a c-di-GMP effector system that controls biofilm in Bordetella bronchiseptica.
Autor/es:
AMBROSIS, NICOLÁS; SISTI, FEDERICO; FERNANDEZ, JULIETA
Reunión:
Congreso; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2014
Resumen:
Bordetella
bronchiseptica
isa
respiratory pathogenic bacterium that forms
biofilm-like structures. Previously, we
determined that c-di-GMP levels modified ability to form biofilm.
However, the bacterial factors involved in biofilm formation has not
been yet determined.
In Pseudomonas
fluorescens a
c-di-GMP effectors system controls biofilm formation. LapA is a
surface adhesin and its binding to the cell surface is controlled by
LapD, in response to c-di-GMP. LapD promotes biofilm formation
through LapA accumulation. LapG cleaves LapA, freeing the adhesin and
preventing biofilm. Accordingly, when LapG is overexpressed or LapD
is absent biofilm is prevented.
Homologous
to Lap proteins were identified in B.
bronchiseptica.
When LapGBbwas
overexpressed less biofilm was formed on abiotic surfaces. More
over, biofilm structures observed by fluorescent microscopy over
glass surface and epithelial cells were significantly different.
Protruding structures typically observed in wild type biofilms were
rarely observed in Bb-LapGBb
.
To
evaluate if LapDBb
is involved in biofilm formation, LapDBb
gene was deleted. Surprisingly, LapDBb
deletion
enhanced biofilm formation in B.
bronchiseptica.
This
results showed that although Lap proteins are widespread among gram
negative bacteria, their mechanisms may be diverse as LapDBb
deletion showed an opposite phenotype than the observed in P.
fluorescens.