INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
The monomeric GTPase ARFA1a locates to large moving vesicles and partially co-localize with RabA2
RIVERO, CLAUDIO; TRAUBENIK, SOLEDAD; ZANETTI, MARÍA EUGENIA; BLANCO, FLAVIO; DALLA VIA, VIRGINIA
Congreso; XV Congreso Latinoamericano XXX Reunión Argentina de Fisiología Vegetal; 2014
Soil bacteria known as rhizobia associate with legume plants, resulting in the formation of nitrogen-fixing nodules in the root. Bacteria are internalized through a tubular structure, the infection thread, which is formed by an inversion of polar growth of root hairs. Formation of infectio structures requires an active vesicle trafficking to transport membrane and cell wall material. Monomeric GTPases participates in all steps of vesicle transport across the cytosqueleton. We have previously shown that RabA2, a monomeric Rab GTPase participates on polar growth root events and rhizobial infection. ARFA1a was identified as a gene co-regulated with RabA2. Localization experiments showed that ArfA1a-RFP localized on relatively large punctuate structures of 2-3 µm located in the cytoplasm and the membrane. These moving structures resemble the subcellular localization previously described for ArfA1c in Arabidopsis thaliana. Moreover, localization was unaffected after rhizobia inoculation and after BFA treatment. Agroinfiltracion of Nicotiana benthamina leaves showed that GFP-RabA2 partially co-localized with ArfA1a-RFP . Co-expression of ArfA1a-GFP with markers of known subcelullar organelles will help to elucidate the identity of structures labeled by ArfA1a.