INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
?Role of GR1+ cells in experimental murine leptospirosis?
PRÊTRE G, OLIVERA N, CÉDOLA M, ALBERDI L, GÓMEZ RM
Congreso; 7th. Annual Scientific Meeting of International Leptospirosis Society (ILS); 2011
International Leptospirosis Society
The role played by the innate immune response system in the pathogenesis of leptospirosis is still unclear. Depletion of specific immune cells has been successfully used to understand their roles in the immune response. The lack of animals "knockout" in neutrophils or monocytes has demanded the use of antibodies for depletion of these cells in order to investigate their role in experimental animal models. One of these antibodies is RB6-8C5, which has been originally described by its ability to bind to granulocyte receptor-1 (Gr-1) and is capable not only to deplete neutrophils but also monocytes and a subset of CD8+ T cells. In this study, 3-week-old male C3H/HeJ mice were treated or not with RB6-8C5 antibody or with an antibody against the major neutrophil chemokine receptor CXCR2. Animals were then infected with the virulent strain L1-130 of Leptospira interrogans serovar Copenhageni. At 14 days post-infection (pi), several parameters were studied including survival, bacterial burden in kidneys determined by q-PCR, degree of tissue injury by histopathological analysis and serological levels of IgM and IgG anti-leptospiral antibodies measured by ELISA. Our results showed that mice treated with RB6-8C5 antibody did not manifest differences in mortality and morbility but exhibited increased bacterial burden in kidneys, aggravated tubulointerstitial nephritis and higher humoral response. In contrast, mice treated with CXCR2 antibody did not show significant differences when compared to untreated infected animals. These results indicate that Gr1+ cells play an important role in leptospirosis.