IN VITRO AND IN VIVO CHARACTERIZATION OF Bordetella bronchiseptica LIPOPOLYSACCHARIDE DEFECTIVE MUTANTS

CORDERO, ANDRES; FERNANDEZ, JULIETA; HOZBOR, DANIELA; SISTI, FEDERICO

Potrero de los Funes, San Luis

Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011

Bordetella bronchiseptica is a respiratory pathogen of differentmammals, including humans. The lipopolysaccharide (LPS) of thispathogen plays an important role in bacterial host interaction notonly as adhesin but also in protection.Here we describe two genes, BB3398 and BB3394,that potentiallycodifiy to known glycoyltransferases involved in the biosynthesis ofthe B. bronchiseptica core oligosaccharide. By insertionallyinactivating these genes and studying the resulting LPS structures,we show that BB3398 presented a deep rough phenotype in SDSPAGEwhile BB3394 mutant profile was similar to parental LPS. Inaddition, we demonstrate that mutations in both genes affect thesensivity to bactericidal activity of mice naïve sera and to detergent.Furthermore, we found that inactivation of the responsibleglycosyltransferases reduces bacterial colonization in mice. Theseresults represent another evidence showing the relevance of thecomplete structure of LPS in bacterial host interaction.