Mucosal innate response stimulation induced by lipopolysaccharide protects against Bordetella pertussis colonization

ERREA AGUSTINA; MORENO GRISELDA; SISTI FEDERICO; FERNÁNDEZ JULIETA; RUMBO MARTIN; HOZBOR DANIELA

MEDICAL MICROBIOLOGY AND IMMUNOLOGY

SPRINGER

Año: 2010 vol. 199 p. 103 - 108

0300-8584

Non-speciWc enhancement of the airwaysinnate response has been shown to impair lung infections inseveral models of infection such diverse as inXuenza A,Streptococcus pneumoniae, and Aspergillus niger. Our aimwas to evaluate whether a similar event could operate in thecontext of Bordetella pertussis respiratory infection, notonly to enrich the knowledge of host–bacteria interactionbut also to establish immunological basis for the developmentof new control strategies against the pathogen. Usinga B. pertussis intranasal infection model and coadministrationof diVerent TLR agonists at the moment of the infection,we observed that the enhancement of innate responseactivation, in a TLR4-dependent way, could eYcientlyimpair B. pertussis colonization (P < 0.001). While LPSfrom diVerent microbial sources were equally eVective inpromoting this eVect, Xagellin and poly I:C coadministration,in spite of inducing expression of innate responsemarkers TNF
, CXCL2, CXCL10 and IL6, was not eVectiveto prevent B. pertussis colonization. Our resultsindicate that during the early stage of infection, speciWcanti-microbial mechanisms triggered by TLR4 stimulationare able to impair B. pertussis colonization. These Wndingscould complement our current view of the role of TLR4-dependent processes that contribute to anti-pertussis immunity.