IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
Genetic diversity among viruses associated with sugarcane mosaic disease in Tucuman, Argentina
Autor/es:
M.F. PERERA, M.P. FILIPPONE, C. J., RAMALLO, M.I. CUENYA, M.L. GARCÍA, L.D. PLOPER, F. GARCÍA ARENAL, AND A.P. CASTAGNARO
Revista:
PHYTOPATHOLOGY
Editorial:
AMER PHYTOPATHOLOGICAL SOC
Referencias:
Año: 2009 vol. 99 p. 38 - 49
ISSN:
0031-949X
Resumen:
Sugarcane leaves with mosaic symptoms were collected in 2006–07 in Tucumán (Argentina) and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) restriction fragment length polymorphism (RFLP) and sequencing of a fragment of the  Sugarcane mosaic virus (SCMV) and  Sorghum mosaic virus (SrMV) coat protein (CP) genes. SCMV was detected in 96.6% of samples, with 41% showing the RFLP profile consistent with strain E. The remaining samples produced eight different profiles that did not match other known strains. SCMV distri-bution seemed to be more related to sugarcane genotype than to geographical origin, and sequence analyses of CP genes showed a greategenetic diversity compared with other studies. SrMV was detected in63.2% of samples and most of these were also infected by SCMVindicating that, unlike other countries and other Argentinean provinceswhere high levels of co-infection are infrequent, co-existence is commonin Tucumán. RFLP analysis showed the presence of SrMV strains M(68%) and I (14%), while co-infection between M and H strains wapresent in 18% of samples. Other SCMV subgroup members and theSugarcane streak mosaic virus (SCSMV) were not detected. Our resultsalso showed that sequencing is currently the only reliable method toassess SCMV and SrMV genetic diversity, because RT-PCR-RFLP maynot be sufficiently discriminating.