IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses.
Autor/es:
NAKAUCHI M, FUKUSHI S, SAIJO M, MIZUTANI T, URE AE, ROMANOWSKI V, KURANE I & MORIKAWA S.
Revista:
CLINICAL VACCINE IMMUNOLOGY
Editorial:
American Society for Microbiology (ASM)
Referencias:
Lugar: Washington DC; Año: 2009 vol. 16 p. 1132 - 1138
ISSN:
1556-679X
Resumen:
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Junin
virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus
are members of New World arenavirus clade B and are the etiological agents of
viral hemorrhagic fevers that occur in South America. In this study, we
produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid
protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these
MAbs was examined by enzymelinked immunosorbent assay (ELISA), indirect
immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we
developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9,
JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or
E-4-2, the Ags of all human pathogenic South American arenaviruses were
detected. The combined use of these Ag capture ELISA systems in the present
study may be useful for the diagnosis of acute-phase viral hemorrhagic fever
due to infection by a South American arenavirus.