Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B síndromes

NORMA COSTA; AGUSTINA DE FRANCESCO,; MARIA LAURA GARCIA

TRANSGENIC RESEARCH

SPRINGER

Lugar: Berlin; Año: 2016

0962-8819

Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious andwidespread citrus disease. Two syndromes of psorosis, PsA and PsB, havebeen described. PsB is the most aggressive and rampant form. Previously, weobtained Pineapple sweet orange plants transformed with a hairpin constructderived from the CPsV coat protein gene (ihpCP). Some of thethese plantswere resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. Inthis study, we found that expression of the ihpCP transgene and siRNAproduction in lines ihpCP-10 and -15 were stable with time and propagation.In particular, line ihpCP-15 has been resistant for more than 2 years, evenafter re-inoculation. The Lines ihpCP plants were also resistant against aheterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15manifested complete resistance while line ihpCP-10 was tolerant to the virus,although with variable behaviour, showing delay and attenuation in PsBsymptoms. These lines are promising for a biotech product aimed ateradicating psorosis