Reporter High FiveTM XXL-GFP Cell line Induced by AcMNPV-DsRED Baculovirus Infection

HAASE S, LÓPEZ MG, TABOGA O, ROMANOWSKI V

CLONING and TRANSGENESIS

OMICS Group

Año: 2015 vol. 4 p. 102 - 102

"Clinical Image"The images correspond to a transgenic cell line derived from the lepidopteran cell line High FiveTM that was engineered to express GFP under the control of the AcMNPV (Autographa californica Multiple Nucleopolyhedrovirus) polyhedrin promoter.This transgenic cell line (named XXL-GFP) was infected with an AcMNPV that expresses the red fluorescent protein (DsRED) under the control of the polyhedrin promoter. Thus, red fluorescence acts as a marker of the baculovirus infection and green fluorescence acts as a marker of the cell line transgene expression. Images a-b and c-d correspond to the same frame with Red (a and c) or Green (b and d) excitation fluorescence filters.