IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae
Autor/es:
BIEDMA, M.E.; SALVADOR, R; FERRELLI, ML; SCIOCCO-CAP, A; ROMANOWSKI, V
Revista:
BIOLOGICAL CONTROL
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 91 p. 17 - 21
ISSN:
1049-9644
Resumen:
The bean shoot borer Epinotia aporema Wals. (Lepidoptera: Tortricidae) and the velvet bean caterpillar Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) are key pests of soybean and other legume crops in South America. They are often found simultaneously in certain regions. Anticarsiagemmatalis nucleopolyhedrovirus (AgMNPV) is widely used to control A. gemmatalis. More recently, E. aporema granulovirus (EpapGV) has been characterized and evaluated as a bioinsecticide for E. aporema. In order to increase its potential use and to design optimized strategies for the management of lepidopteran pests, we evaluated the interaction between EpapGV and AgMNPV. Bioassays conducted on third instar A. gemmatalis larvae fed with mixtures of viral occlusion bodies (OBs) showed a decrease in the median survival time (from 7.7 days with a dose of 50 AgMNPV OBs/larva to 5.7 days with 50 AgMNPV + 6000 EpapGV OBs/larva). In larvae fed with the viral mixtures, only AgMNPV DNA was detected by PCR. A. gemmatalis peritrophic membranes (PMs) examined by SDS-PAGE and scanning electron microscopy showed signs of damage. Notably, we found the presence ofspheroidal bodies associated with damaged areas in the PMs of larvae fed with EpapGV but not in those that were given AgMNPV alone. These results suggest that EpapGV increases the virulence of AgMNPV, and that the use of formulations including both viruses might be a valuable tool for pest management.