IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
The coat protein and the 54K protein of Citrus psorosis virus are localized in the cytoplasm of Citrus sinensis (sweet orange) and Chenopodium quinoa plants
Autor/es:
PEÑA EJ; ZANEK MC; REYES CA; GRAU O; GARCIA ML
Revista:
JOURNAL OF GENERAL VIROLOGY
Editorial:
The Charlesworth Group
Referencias:
Lugar: Marlborough ; Año: 2008
ISSN:
0022-1317
Resumen:
Citrus psorosis virus (CPsV) is the causal agent of
a serious disease affecting citrus trees in many countries, principally in Argentina and Uruguay. CPsV is the type member of
genus Ophiovirus, which is poorly
characterized. The virus is tripartite and its genome consists of three ssRNAs
of negative polarity. RNA 1 codifies for two ORFs, a polypeptide of unknown
function (24K protein), and the putative RNA-depended RNA polymerase (280K
protein). RNA 2 codes for a polypeptide of unknown function (54K protein), and
RNA 3 the coat protein (48K protein or CP). Attemps to localize the virus in
microscopic sections of infected cells has been unsucessful probably because
the low amonut of particles and the thin structure they have. In order to
investigate the subcellular localization and the interaction of the viral
proteins, we have generated polyclonal antisera to detect them and applied
different methodologies and strategies to localized the CP and the 54K protein.
By confocal microspcopy and subcellular fractionation in infected citrus and
herbaceos hosts both proteins were localizaed in the cytoplasm. Using transient
and stable expression in transgenic plants and GFP-fusioned CP and 54K
proteins, the interaction between them has been studied. All the results
indicates that these proteins have cytoplasmic localization as well as probably
the virus particles.