IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
The coat protein and the 54K protein of Citrus psorosis virus are localized in the cytoplasm of Citrus sinensis (sweet orange) and Chenopodium quinoa plants
Autor/es:
PEÑA EJ; ZANEK MC; REYES CA; GRAU O; GARCIA ML
Revista:
JOURNAL OF GENERAL VIROLOGY
Editorial:
The Charlesworth Group
Referencias:
Lugar: Marlborough ; Año: 2008
ISSN:
0022-1317
Resumen:
Citrus psorosis virus (CPsV) is the causal agent of a serious disease affecting citrus trees in many countries, principally in Argentina and Uruguay. CPsV is the type member of genus Ophiovirus, which is poorly characterized. The virus is tripartite and its genome consists of three ssRNAs of negative polarity. RNA 1 codifies for two ORFs, a polypeptide of unknown function (24K protein), and the putative RNA-depended RNA polymerase (280K protein). RNA 2 codes for a polypeptide of unknown function (54K protein), and RNA 3 the coat protein (48K protein or CP). Attemps to localize the virus in microscopic sections of infected cells has been unsucessful probably because the low amonut of particles and the thin structure they have. In order to investigate the subcellular localization and the interaction of the viral proteins, we have generated polyclonal antisera to detect them and applied different methodologies and strategies to localized the CP and the 54K protein. By confocal microspcopy and subcellular fractionation in infected citrus and herbaceos hosts both proteins were localizaed in the cytoplasm. Using transient and stable expression in transgenic plants and GFP-fusioned CP and 54K proteins, the interaction between them has been studied. All the results indicates that these proteins have cytoplasmic localization as well as probably the virus particles.