Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection

BOTTERO DANIELA; GAILLARD MARÍA EMILIA; ERREA AGUSTINA; MORENO GRISELDA; ZURITA MARÍA EUGENIA; PIANCIOLA, LUIS; RUMBO MARTÍN; HOZBOR DANIELA

VACCINE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2013 vol. 31 p. 5262 - 5268

0264-410X

Bordetella parapertussis, a close related species of B. pertussis, can also cause the disease named pertussis or whooping cough. The number of cases caused by this related pathogen has risen sustained in the last years. The widely used cellular (wP) or acellular (aP) pertussis vaccines have little or no efficacy against B. parapertussis. In an effort to devise an effective acellular vaccine against B. parapertussis infection, outer membrane vesicles (OMVs) were obtained from B. parapertussis. Proteomic analysis of the resulting OMVs, designated OMVsBpp, evidenced the presence of several surface immunogens including pertactin. The characterized OMVsBpp were used in murine B. parapertussis intranasal challenge model to examine their protective capacity when administered by systemic route. Immunized BALB/c mice were challenged with sublethal doses of B. parapertussis. Significant differences between immunized animals and the negative control group were observed (p