IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America.
Autor/es:
BOTTERO D.; GAILLARD M.E.; BASILE, A.L.; FRITZ, M.; HOZBOR D.
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2012
ISSN:
1364-5072
Resumen:
Aims:  Characterize Bordetella pertussis vaccine strains in comparison with current circulating bacteria. Methods and Results:  Genomic and proteomic analysis of Bp137 was performed in comparison with other vaccine strains used in Latin America (Bp509, and Bp10536) and with the clinical Argentinean isolate Bp106. Tohama I strain was used as reference strain. PFGE and pertussis toxin promoter (ptxP) sequence analysis revealed that Bp137 grouped with Bp509 in PFGE Group III, and contain ptxP2 sequence. Tohama I (Group II) and Bp10536 (Group I) contain ptxP1 sequence, while Bp106 belongs to a different PFGE cluster and contains ptxP3. Surface protein profiles diverged in at least 24 peptide subunits among the studied strains. From these 24 differential proteins, Bp10536 shared the expression of 10 proteins with Tohama I and Bp509, but only 3 with Bp137. In contrast, 7 proteins were detected exclusively in Bp137 and Bp106.Conclusions: Bp137 showed more features in common with the clinical isolate Bp106 than the other vaccine strains here included. Significance and Impact of the Study:  The results presented show that the old strains included in vaccines are not all equal among them. These findings together with the data of circulating bacteria should be taking into account to select the best vaccine to be included in a national immunization program. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.