IBBM   21076
INSTITUTO DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Unidad Ejecutora - UE
artículos
Título:
Effect of temperature on RNA silencing of a negative stranded plant virus: citrus psorosis virus
Autor/es:
VELÁZQUEZ, K., RENOVELL, A, COMELLAS, M. LAURA GARCIA, M.L. NAVARRO, L, PINA. J.A., MORENO, P. AND J. GUERR
Revista:
PLANT PATHOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 59 p. 982 - 990
ISSN:
0032-0862
Resumen:
Citrus
psorosis virus (CPsV), genus Ophiovirus,
causes a bark scaling disease of citrus. CPsV virions are kinked filaments
with
three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The
effect of temperature on symptom
expression,
virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus
sinensis) graft-inoculated
with
three different CPsV isolates and grown in a glasshouse at 26⁄ 18_C or 32⁄26_C (day⁄ night). Most plants kept in the
cooler
glasshouse showed a shock reaction in the first flush with shoot necrosis, and
then moderate to intense chlorotic flecking
and
spotting in young leaves, whereas plants incubated at 32⁄ 26_C did not exhibit shoot necrosis,
and young leaf symptoms
were
milder. Virus titre estimated by ELISA and by northern and dot blot
hybridization paralleled symptom intensity,
with
significantly higher virus accumulation in plants incubated at 26⁄ 18_C. The amount of CPsV-derived small
RNAs
(CPsV-sRNAs)
slightly increased at 32⁄26_C, with the ratio of CPsV-sRNA⁄vRNA being higher at 32 ⁄ 26_C than at
26⁄18_C. These results suggest that (i) CPsV
infection induces RNA silencing in citrus plants, (ii) symptom intensity is
associated
with
virus accumulation, and (iii) temperature increase enhances the RNA silencing
response of citrus plants and
decreases
virus accumulation.