Genome sequence of Bradyrhizobium yuanmingense strain P10 130, a highly efficient nitrogen-fixing bacterium that could be used for Desmodium incanum inoculation

ALBICORO, FRANCISCO JAVIER; FORNASERO, LAURA VIVIANA; VACCA, CAROLINA; KALINOWSKI, JÖRN; DEL PAPA, MARÍA FLORENCIA; CASTELLANI, LUCAS GABRIEL; ZUBER, NICOLÁS EMILIO; CAFIERO, JUAN HILARIO; LAGARES, ANTONIO; TONIUTTI, MARÍA ANTONIETA; LÓPEZ GARCÍA, SILVINA LAURA; VERA, LEDA MAILÉN; WINKLER, ANIKA; TORRES TEJERIZO, GONZALO ARTURO

GENE

ELSEVIER SCIENCE BV

Año: 2021 vol. 768

0378-1119

Strain P10 130, an isolated Bradyrhizobium strain from Argentina which promotes the growth of the leguminous plant Desmodium incanum by different mechanisms was previously selected as the best candidate for D. incanum inoculation based on broader selective criteria. A close relationship between this strain and B. yuanmingense was determined by MALDI BioTyper identification and 16S rRNA gene phylogenetic analysis. This study aimed to analyse the genome sequence of B. yuanmingense P10 130 in order to deepen our knowledge regarding its plant growth-promoting traits and to establish its phylogenetic relationship with other species of Bradyrhizobium genus. The genome size of strain P10 130 was estimated to be 7.54 Mb that consisted of 65 contigs. Genome Average Nucleotide Identity (ANI) analysis revealed that B. yuanmingense CCBAU 10071 T was the closest strain to P10 130 with ca. 96% identity. Further analysis of the genome of B. yuanmingense P10 130 identified 20 nod/nol/NOE, 14 nif/18 fix, 5 nap/5 nor genes, which may be potentially involved in nodulation, nitrogen fixation, and denitrification process respectively. Genome sequence of B. yuanmingense P10 130 is a valuable source of information to continue the research of its potential industrial production as a biofertilizer of D. incanum.