IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of dietary fish oil and vitamin E on DNA damage in dogs undergo training.
Autor/es:
CORRADA Y; RELLING A; PELLEGRINO F; SEOANE A; RISSO A; NIKOLOFF N
Reunión:
Congreso; Annual Meeting and Trade Show, American Society of Animal Science; 2017
Institución organizadora:
American Society of Animal Science
Resumen:
Effect of dietary fish oil and vitamin E on DNA damage in dogs undergo trainingThe aim of the present study was to evaluate the effect of dietary supplementation with fish oil (FO) alone or FO and vitamin E (VE) on DNA damage and serum VE concentration in dogs undergoing training. Using a replicate 3x3 Latin square design: 1) a control diet (CG); 2) the same diet supplemented with 54 mg FO/kg BW (FG); and 3) a similar diet plus FO (54mg/kg BW) and 400 mg VE (FEG) per day for 60 d. Blood samples were collected on d0, 30 and 60. Deoxyribonucleic acid damage was measured using a single-cell gel electrophoresis assay in peripheral whole blood leukocytes. Deoxyribonucleic acid damage was classified in 4 classes (I = undamaged; II = minimum damage, III = medium damage and IV = maximum damage). Dogs were trained on a treadmill at 8 km/h and 7.5% slope twice a week for 60 d. Each session lasted 30 min. Data were analyzed usin PROC MIXED of SAS (9.4 SAS Inst. Inc., Cary, NC). Treatment time, and their interaction were considered fixed variables and dog and period were considered random. The SLICE option of SAS was used for mean separation when there was a treatment x time interaction. On d 30, there was a significant increase in DI in FG compared with CG and FEG (p