Effect of fish oil and vitamin e on sperm lipid peroxidation in dogs

PELLEGRINO FJ; MARMUNTI M; RISSO A; PELOSI J; PALACIOS A; PLAZA L; GAVAZZA M

Ciudad Autónoma de Buenos Aires

Exposición; VI Jornadas de Jóvenes Investigadores; 2016

Facultad de Ciencias Veterinarias, Universidad de Buenos Aires

Mammalian sperm contains a high proportion of polyunsaturated fatty acids sensitive to lipid peroxidation (LP). Objective. Evaluate the effects of dietary fish oil (FO) and vitamin E (VE) supplementation on sperm sensitivity to LP in dogs. Using a 3x3 Latin square design, five male dogs were allocated into three groups and fed a control diet (C), C supplemented with 54 mg fish oil/Kg metabolic body weight per day (FO), and FO plus 400 mg VE per day (FOE) for 60 days. Semen samples were collected on days 0 and 60. Lipid peroxidation was initiated by adding ascorbate?Fe++ to semen samples and evaluated using chemiluminescence (CL) (counts per minute/mg of protein). Fatty acid profile was determined by gas chromatography. Data were analyzed using SAS. When the control and ascorbate?Fe++ dependent samples were compared, a significant increase in CL was observed. On day 0, total CL increased in all groups in the peroxidised samples (with ascorbate?Fe++) (p