EFFECT OF FISH OIL AND ALPHA TOCOPHEROL ON SPERM LIPID PEROXIDATION IN DOGS

GAVAZZA M; RELLING A E; A RISSO; PALACIOS A; MARMUNTI M; PELLEGRINO F

Chicago

Simposio; Growing Science in Pet Nutrition; 2016

Waltham International Nutritional Science Symposium

Introduction Mammalian sperm contains a high proportion of polyunsaturated fatty acids (PUFA) sensitive to lipid peroxidation (LP). The objective of this study was to evaluate the effects of dietary fish oil and vitamin E (VE) supplementation on sperm sensitivity to LP in dogs. Materials and methods Using an incomplete replicate 3x3 Latin square design, five male dogs were randomly allocated into three groups and fed a control diet (C), C supplemented with 54 mg fish oil/Kg metabolic body weight per day (FO), and FO plus 400 mg VE per day (FOE) for 60 days. Semen samples were collected on days 0 and 60. Lipid peroxidation was initiated by adding ascorbate?Fe++ to semen samples and evaluated using chemiluminescence (CL) (counts per minute/mg of protein). Fatty acid profile was determined by gas chromatography. Data were analyzed using a mixed model with repeated measures (SAS 9.0). Results When the control and ascorbate?Fe++ dependent samples were compared, a significant increase in the light emission (CL) was observed. On day 0, total CL increased in all groups from 429.4±40.10 cpm in control samples to 990.5±87.19 cpm in the peroxidised samples (with ascorbate?Fe++) (p