IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Food safety evaluation of a soft drink and caramel color additive (e150d)
Autor/es:
PILAR ALAVAREZ; ROCIO MERINAS-AMOS; ANGELES ALONSO MORAGA; MARCOS MATEO FERNANDEZ; TANIA MERINAS-AMO; LUIS PEREZ; ZAHIRA FERNANDEZ BEDMAR; FERNANDO VALENZUELA; ANTONIO VELAZCO; SEBASTIÁN DEMYDA PEYRÁS
Lugar:
Sevilla
Reunión:
Conferencia; 52nd Congress of the European Societies of Toxicology; 2016
Institución organizadora:
European Societies of Toxicology
Resumen:
Schools and local communities make the effort to reduce the consumption of sugary beverages since they are not part of a balanced diet. Despite this fact, Pepsi-Cola is one of the most consumed soda drinks. Therefore, studies are needed to evaluate the biological effects of lyophilised Pepsi-Cola (PEP) and a main additive like caramel color E-150d (CAR). We performed in vivo toxicity,antitoxicity and lifespan assays using Drosophila melanogaster; and in vitro cytotoxicity, DNA fragmentation, methylation status and comet assays using HL-60 promyelocytic cell line. Results showed that PEP is toxic at the highest assayed concentration (110 mg/ml) and protects the individuals against the toxicant hydrogen peroxide at the lowest one (0.86 mg/ml). CAR was toxic at all assayedconcentrations. Regarding lifespan assays, PEP showed the same effect as negative control whereas CAR decreased the longevity of flies. According to in vitro assays, PEP and CAR were cytotoxic reaching IC50 at 25 mg/ml and 1 mg/ml respectively. However, none of the tested compounds were able to induce DNA internucleosomal fragmentation. PEP increased the repetitive elementsmethylation status and induced neither single nor double strand DNAbreaks in HL-60 cells. In conclusion,wedemonstrate the safety of PEP in the Drosophila model, and its antioxidant in vivo activity at the lowest assayed concentration. Although PEP and CAR show a chemopreventive property, it is not due to proapoptotic mechanisms. CAR could not be considered as a safe additive sinceit is toxic and decrease the lifespan expectancy in the Drosophila model.