IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of increasing zinc concentrations during in vitro culture of bovine embryos
Autor/es:
ANCHORDOQUY JM; ANCHORDOQUY JP; SIRINI MA; TESTA JA; CÉTICA PD; FURNUS C.
Lugar:
Chascomus
Reunión:
Jornada; XVI Jornadas Anuales de la Sociedad Argentina de Biología; 2014
Institución organizadora:
Sociendad Argentina de Biología
Resumen:
In previous studieswe demonstrated that zinc (Zn) supplementationduring in vitro maturation (IVM) improves the
quality of bovine oocytes and consequently their competence for developing to blastocyst
stage. However, the influence of Zn supplementation during in vitro culture (IVC) of preimplantational bovine embryos has not
been yet investigated. The aim of this study was to evaluate the effect of Zn addition
during IVC. For this purpose, cumulus oocyte-complexeswere obtained from an
abattoir, matured in TCM 199 medium with 10% BFSfor 24 h and then in vitro fertilized. After IVF,
presumptive cigoteswere cultured during 8 Days in mSOF with: 0; 0.4; 0.8 and
1.2µg/mL Zn. A completely randomized block designs were used and statistical
models included the random effects of block and the fixed effect of treatment. Cleavage and blastocyst rates were analyzed with logistic
regression by using the GENMOD procedure (SAS Institute,
Cary, NC) with binomial distribution. Data was expressed as
percentages. There were no differences in both cleavage rates (59; 64.7; 67.5 and 70 % for 0; 0.4; 0.8 and 1.2
µg/mL Zn) and blastocyst rates (15.7; 20.8; 21.8 and 17.4 for 0, 0.4, 0.8, and
1.2 µg/mL Zn) when Zn was added to IVC medium at any concentration.In conclusion, Zn addition during IVC ofbovine embryos did not modifycleavage
and blastocyst rates.