Assessment of biodiversity in Chilean cattle using the distribution of MHC class II BoLA-DRB3 allele.

TAKESHIMA S-N; MIYASAKA T.; MATSUMOTO Y.; XUE G.; DE LA BARRA DIAZ V; ROGBERG MUÑOZ A.; GIOVAMBATTISTA G.; ORTIZ M.L.; OLTRA J.; KANEMAKI M.; ONUMA M.; AIDA Y.

Xi´an

Conferencia; 34rd Conference of the International Society of Animal Genetics; 2014

ISAG

Bovine leukocyte antigens (BoLA) are extensively used as markers for bovine disease and immunological traits. In this study, we determined BoLA-DRB3 allele frequencies in 888 cattle from ten groups, including seven cattle breeds and three crossbreds: 99 Red Angus, 100 Black Angus, 81 Chilean Wagyu, 49 Hereford, 95 Hereford × Angus, 71 Hereford × Jersey, 20 Hereford × Overo Colorado, 113 Holstein, 136 Overo Colorado, and 124 Overo Negro cattle. Forty-six BoLA-DRB3 alleles were identified in the ten Chilean cattle groups. Each group had 12 to 29 different BoLA-DRB3 alleles. The highest number of alleles (29 alleles) was detected in Overo Negro, which is considered in Chile as an ?Old type? European Holstein Friesian descendant. By contrast, 21 alleles were detected in Holstein, which is considered as a ?Present type? Holstein Friesian cattle. Chilean cattle groups were compared by phylogenetic and principal component analysis (PCA) using four Japanese breeds as an out group. The phylogenetic tree showed that Red Angus and Black Angus were in the same clade, crossbreeds were positioned close to the original breeds, and Holstein from Chile was positioned in the same clade as Holstein in Japan. Therefore, this tree provided a good explanation of breed history. The tree also showed that Overo Negro was close to Holstein, consistent with the historical data that Overo Negro is the ?Old type? Holstein Friesian cattle. PCA showed that the Chilean breed originated in Britain (Angus, Hereford, and Jersey) and was distinct from breeds originating in the European continent (Holstein, Overo Colorado, and Overo Negro) at the point view of first principal component mainly computed from the frequencies of 16 BoLA-DRB3 alleles. This allelic information will be important for investigating the relationship between MHC and disease.