IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Advances on gen-specific genotyping method for equine MHC polymorphisms
Autor/es:
DIAZ S.; POSIK D.M.; PERAL GARCIA G.; GIOVAMBATTISTA G.
Lugar:
Tokio
Reunión:
Simposio; 9th International Symposium of Veterinary Immunology.; 2010
Institución organizadora:
International Society of Veterinary Immunology.
Resumen:
MHC genes had been extensively used for population genetic and phylogenetic studies, as well as, for evaluated association with immune and infection diseases. For these purposes, it is necessary to develop gene specific genotyping methods. In equine, three functional DRB genes, containing 20 exon 2 alleles, have been reported. However, these alleles were not assigned to a specific DBR gene. Furthermore, there are not available gene specific genotyping assays. In order to develop ELA-DRB gene specific genotyping methods, DNA sequences between promoter and exon 3 regions were analyzed in 30 samples belonged to seven horse breeds. Reported sequences of DRB1, DRB2, DRB3, DRB5 and DRB pseudogene from Equines Genome sequences were aligned and compared in order to design primers specific for each locus that they were located at exon 2 and intron 2 boundaries. Exon 2 variants were identified by PCR-RFLP and DNA sequencing. The main obtained results allowed to: i) identify exon 2 DRB2 and DRB3 alleles; ii) assign alleles to specific locus; iii) detect DRB pseudogene alleles; iv) describe microsatellite sequences located in exon 2-intron 2 boundary, and use this information to development a DRB-STR typing method; v) detect new DRB exon 3 polymorphisms; vi) develop DRB gene specific methodology. The obtained results could allow a more accurate ELA-DRB genotyping typing for population characterization and phylogenetic investigations, and study association between DRB polymorphism and immune and infectious diseases.