IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Extended Phylogeny of the Equine Arteritis Virus Sequences including South-American Sequences
Autor/es:
METZ GE; MARTIN OCAMPOS G; SERENA MS; GAMBARO S; NOSETTO EDGARDO OMAR; ECHEVERRIA MG
Revista:
INTERVIROLOGY
Editorial:
KARGER
Referencias:
Lugar: Leipzig; Año: 2010
ISSN:
0300-5526
Resumen:
Objective: To perform genetic analysis of the ORF5 of equine
arteritis virus (EAV) may provide new insights into the genetic
evolution and origin of the Argentinean EAV sequences.
Methods: A total of 76 sequences were analyzed by
neighbor joining (NJ), maximum parsimony and maximum
likelihood algorithms. The analysis of the selective pressures
was performed using the Tajimas test. Results: The trees
showed similar topologies. Two clades were identified: the
first clade was formed by strains isolated mainly from a donkey,
whereas the second clade presented four large groups
from different geographic regions exclusively from Equus caballus
. In this clade, we identified a group formed by South
African and another one by South American and European
sequences. In the latter, the monophyletic group was formed
by seven Argentinean sequences. In the NJ tree, we identified
a group formed by six Argentinean sequences. The Tajimas
test showed a D value of 1.73663, indicating that the
sequences analyzed follow a neutral evolution model. Conclusion:
We concluded that the Argentinean sequences have a paraphyletic origin and that the fixation of point mutation
might follow the neutral model evolution; however,
we identified purifying pressures that may be involved in the
differentiation of the EAV sequences.