IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Doramectin induced cytotoxic and genotoxic effects on bovine peripheral lymphocytes and cumulus cells in vitro
Autor/es:
NIKOLOFF N; SEOANE A. I.; ANCHORDOQUY J.P; PADULA, G; ANCHORDOQUY J.M.; GAMBARO, ROCÍO; FURNUS C.C.
Revista:
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH. PART B - PESTICIDES, FOOD CONTAMINANTS, AND AGRICULTURAL WASTES
Editorial:
TAYLOR & FRANCIS INC
Referencias:
Lugar: Londres; Año: 2019 vol. 54 p. 147 - 154
ISSN:
0360-1234
Resumen:
The effect of doramectin (DOR) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PL) and cumulus cells (CC). The cytotoxicity and genotoxicity of DOR were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, single cell gel electrophoresis assay (SCGE) and cytokinesis-block micronucleus cytome (CBMN Cyt) assay. Both cells were treated with three concentrations of DOR (20, 40, 60 ngmL?1) for 24 h. The results obtained from PL demonstrated that DOR was able to induce cytotoxic effect and DNA damage with all concentrations tested. Additionally, DOR increased micronuclei (MNi) frequency and nuclear buds (NBuds) with 20, 40, 60 ngmL?1, and nucleoplasmic bridges (NPBs) only with 40 ngmL?1. On the other hand, the three concentrations of DOR were not able to induce cytotoxic effect and DNA damage using SCGE in the bovine CC. Nevertheless, the two higher concentrations of DOR (20, 40 mg mL?1) significantly increased the frequency of micronucleus formation in bovine CC. These results represent the first experimental evidence of genotoxic and cytotoxic effects exerted by DOR on bovine PL and CC.