IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Restriction Fragment Length Polymorphism analysis of Argentinean Field Isolates of Suid Herpesvirus Type 1 and Molecular Characterization of the Major Epitope domain of Glycoprotein E
Autor/es:
SERENA MARÍA SOLEDAD; METZ G. E.; MARTIN OCAMPOS G; MORTOLA E; ECHEVERRÍA MARÍA
Revista:
REVISTA ARGENTINA DE MICROBIOLOGíA
Editorial:
ASOCIACION ARGENTINA MICROBIOLOGIA
Referencias:
Año: 2009
ISSN:
0325-7541
Resumen:
The genomic characterization of suid herpesvirus type I (SHV-I) isolates from Argentina was accomplished by restriction pattern analysis using the BamHI, BstEII and XhoI enzymes. Type II genome was described in Argentina only once. This study revealed considerable homogeneity of BamHI endonuclease sites in all the strains analyzed, according to the number and size of the fragments. No deletion of BamHI fragment #7 among the Argentinean isolates suggests that these strains are wild-type. In addition, the main antigenic domain of glycoprotein E of all Argentinean strains, as well as the reference strains and sequences available in GenBank, were characterized. The similarity percent oscillated between 99 and 100%.