IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Effect of increasing zinc sulphate concentration during in vitro maturation of cattle oocytes.
Autor/es:
PICCO SJ; ANCHORDOQUY JM; DE MATOS DE, ANCHORDOQUY JP; SEOANE A; MATTIOLI GA; ERRECALDE AL; FURNUS CC
Revista:
THERIOGENOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2009
ISSN:
0093-691X
Resumen:
The aim of this study was investigate the effect of supplementary zinc during in vitro maturation (IVM) of cattle oocytes. Experiments were designed to evaluate the effect of 0.7 μg/mL, 1.1μg/mL and 1.5 μg/mL Zn during IVM on DNA integrity of cumulus cells by Comet Assay; intracellular total glutathione concentration in oocytes and cumulus cells; and developmental capacity of oocytes matured with different zinc concentrations. Damage of DNA in cumulus cells was low when zinc was added to IVM medium in marginal deficient and adequate concentrations (Control: 67.52 ± Total glutathione concentrations did not differ between 1.1 and 1.5 μg /mL (3.7 ± 0.4 vs 4.0 ± 0.5 pmol/oocytes respectively; (0.5 ± 0.04 nmol x 106cumulus cells in both treatments) but were cells). There was an increase in cleavage rate (Control: 67.16 ± 1.17; 0.7 μg/mL Zn: 73.15 ± 1.15; 1.1 μg/mL Zn: 74.05 ± 1.23; 1.5 μg/mL Zn: 72.76 ± 0.74) when zinc was added to IVM medium at any trol: 17.83 ± 2.15; 0.7 μg/mL Zn: 21.95 ± 0.95; 1.1μg/mL Zn: 27.65 ± 1.61; 1.5 μg/mL Zn: 30.33 ± 2.78) was significantly per blastocyst obtained from oocytes matured with 1.1 and 1.5 μg/mL Zn (P < 0.05) respect to Control (IVM alone) and 0.7 μg/mL Zn. In conclusion, zinc during oocytes maturation can influence intracellular GSH content and DNA integrity of cumulus cells and improve preimplantational embryo development. This study provides evidence that optimal embryo development to blastocyst stage is partially dependent on the presence of adequate zinc concentrations.